Indiana University
* corresponding author † these authors contributed equally
Weisler, E.E., Liao, Q., Ren, Z., Zhang, K.F., Dai, J., Ma, Y., Hardy, G.G., Wang*, X. (2025) ComK-induced cell death is reversed by upregulating the SigB or Spx pathway in Bacillus subtilis. Microbiology Spectrum, 2025 Aug 7:e0161225. doi: 10.1128/spectrum.01612-25. PMID: 40772768
Liao, Q., Brandão, H.B., Ren, Z., Wang*, X. (2025) Replisomes restrict SMC translocation in vivo. Nature Communications. 2025 Aug 4;16(1):7151. doi: 10.1038/s41467-025-62596-y. PMID: 40759902
O'Neal, L.G., Drucker, M.N., Lai, N.K., Clemente, A.F., Campbell, A.P., Way, L.E., Hong, S., Holmes, E.E., Rancic, S.J., Sawyer*, N., Wang*, X., Thrall*, E.S. (2025) The B. subtilis replicative polymerases bind the sliding clamp with different strengths to tune replication processivity and fidelity. Nucleic Acids Research. 2025 Jul 19;53(14):gkaf721. doi: 10.1093/nar/gkaf721. PMID: 40737090
Sanath-Kumar†, R., Rahman†, A., Ren, Z, Reynolds, I.P., Augusta, L., Fuqua, C, J. Weisberg*, A.J., Wang*, X. (2025) Linear dicentric bacterial chromosomes in Agrobacterium tumefaciens natural isolates reveal common constraints for replicon fusion. mBio. 2025 May 20:e0104625. doi: 10.1128/mbio.01046-25. PMID: 40391973.
Mishra, A., Jackson, A., Wang, X., Kearns*, D.B. (2025) The SinR•SlrR heteromer attenuates transcription of a long operon of flagellar genes in Bacillus subtilis. Journal of Molecular Biology. 2025 Apr 3:169123. doi: 10.1016/j.jmb.2025.169123. PMID: 40187681.
Ren†, Z., Way†, L.E., Wang*, X. (2025) SMC translocation is unaffected by an excess of nucleoid associated proteins in vivo. Scientific Reports. 2025 Jan 19;15(1):2447. doi: 10.1038/s41598-025-86946-4. PMID: 39828741
Liao, Q., Wang*, X. (2025) Using Chromosome Conformation Capture Combined with Deep Sequencing (Hi-C) to Study Genome Organization in Bacteria. High Throughput Gene Screening: Methods and Protocols. Methods in Molecular Biology. 2025:2866:231-243. doi: 10.1007/978-1-0716-4192-7_13. PMID: 39546206
Baglivo*, I., Malgieri, G., Roop, R.M. 2nd, Barton, I.S., Wang, X., Russo, V., Pirone, L., Pedone, E.M., Pedone*, P.V. (2024) MucR protein: Three decades of studies have led to the identification of a new H-NS-like protein. Molecular Microbiology. 2024 Apr 15. doi: 10.1111/mmi.15261. PMID: 38619026.
Mishra, A., Hughes, A.C., Amon, J.D., Rudner, D.Z., Wang, X., Kearns*, D.B. (2024) SwrA-mediated multimerization of DegU and an upstream activation sequence enhance flagellar gene expression in Bacillus subtilis. Journal of Molecular Biology. 2024 Feb 15;436(4):168419. doi: 10.1016/j.jmb.2023.168419. Epub 2023 Dec 21. PMID: 38141873
Barton, I.S., Ren, Z., Cribb, C.B., Pitzer, P.E., Baglivo, I., Martin, D.W., Wang*, X., Roop*, R.M. (2023) Brucella MucR acts as an H-NS-like protein to silence virulence genes and structure the nucleoid. mBio. 2023 Dec 19;14(6):e0220123. doi: 10.1128/mbio.02201-23. Epub 2023 Oct 17. PMID: 37847580
Molina†, M., Way†, L.E., Ren, Z., Liao, Q., Guerra, B. Shields, B., Wang*, X., Kim*, H. (2023) A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments. Cell Reports Methods. 2023 Oct 3:100614. doi: 10.1016/j.crmeth.2023.100614. PMID: 37832544
Ren†, Z., Takacs†, C.N., Brandão, H.B., Jacobs-Wagner*, C., Wang*, X. (2023) Organization and replicon interactions within the highly segmented genome of Borrelia burgdorferi. PLOS Genetics. 2023 Jul 26;19(7):e1010857. doi: 10.1371/journal.pgen.1010857. eCollection 2023 Jul. PMID: 37494383
Takacs, C.N., Wachter†, J., Xiang†, Y., Ren†, Z, Karaboja, X, Scott, M., Stoner, M.R., Irnov, I., Jannetty, N., Rosa, P.A., Wang*, X., Jacobs-Wagner*, C. (2022) Polyploidy, regular patterning of genome copies, and alternative control of DNA partitioning in the Lyme disease spirochete. Nature Communications. 2022 Nov 22;13(1):7173. doi: 10.1038/s41467-022-34876-4. PMID: 36450725
Lamanna, M.M., Manzoor, I., Joseph, M., Ye, Z.A., Benedet, M., Zanardi, A., Ren, Z., Wang, X., Massidda, O., Tsui*, H.C.T., Winkler*, M.E. (2022) Roles of RodZ and Class A PBP1b in the Assembly and Regulation of the Peripheral Peptidoglycan Elongasome in Ovoid-Shaped Cells of Streptococcus pneumoniae D39. Molecular Microbiology. 2022 Oct;118(4):336-368. doi: 10.1111/mmi.14969. Epub 2022 Aug 24. PMID: 36001060
Liao†, Q., Ren†, Z., Wiesler, E.E., Fuqua, C., Wang*, X. (2022) A dicentric bacterial chromosome requires XerC/D site-specific recombinases for resolution. Current Biology. 2022 Aug 22;32(16):3609-3618.e7. doi: 10.1016/j.cub.2022.06.050. Epub 2022 Jul 6. PMID: 35797999
Sanchez, S., Snyder, E.V., Wang, X., Kearns*, D.B. (2022) Identification of genes required for swarming motility in Bacillus subtilis using transposon mutagenesis and high-throughput sequencing (TnSeq). Journal of Bacteriology. 2022 Jun 21;204(6):e0008922. doi: 10.1128/jb.00089-22. Epub 2022 May 31. PMID: 35638827
Montero Llopis P., Stephansky R., Wang*, X. (2022) High-throughput imaging of Bacillus subtilis. Methods in Molecular Biology. 2022;2476:277-292. doi: 10.1007/978-1-0716-2221-6_19. PMID: 35635710
Karaboja, X., Wang*, X. (2022) HBsu is required for the initiation of DNA replication in Bacillus subtilis. Journal of Bacteriology. 2022 Aug 16;204(8):e0011922. doi: 10.1128/jb.00119-22. PMID: 35546541
Ren, Z., Liao, Q., Barton, I.S., Wiesler, E.E., Fuqua, C., Wang*, X. (2022) Centromere interactions promote the maintenance of the multipartite genome in Agrobacterium tumefaciens. mBio. 2022 Jun 28;13(3):e0050822. doi: 10.1128/mbio.00508-22. Epub 2022 May 10. PMID: 35536004
Ren, Z., Liao, Q., Karaboja, X., Barton, I.S., Schantz, E.G., Mejia-Santana, A., Fuqua, C., Wang*, X. (2022) Conformation and dynamic interactions of the multipartite genome in Agrobacterium tumefaciens. PNAS. 2022 Feb 8;119(6):e2115854119. doi: 10.1073/pnas.2115854119. PMID: 35101983
Dobihal, G.S., Flores-Kim, J., Roney, I.J., Wang, X., Rudner*, D.Z. (2022) The WalR-WalK signaling pathway modulates the activities of both CwlO and LytE through control of the peptidoglycan deacetylase PdaC in Bacillus subtilis. Journal of Bacteriology. 2022 Feb 15;204(2):e0053321. doi: 10.1128/JB.00533-21. Epub 2021 Dec 6. PMID: 34871030
Brandão†, H.B., Ren†, Z., Karaboja, X., Mirny*, L.A., Wang*, X. (2021) DNA-loop extruding SMC complexes can traverse one another in vivo. Nature Structural & Molecular Biology. 2021 Aug;28(8):642-651. doi: 10.1038/s41594-021-00626-1. Epub 2021 Jul 26. PMID: 34312537
Karaboja, X., Ren, Z., Brandão, H.B., Paul, P., Rudner*, D.Z., Wang*, X. (2021) XerD unloads bacterial SMC complexes at the replication terminus. Molecular Cell. 2021 Feb 18;81(4):756-766.e8. doi: 10.1016/j.molcel.2020.12.027. Epub 2021 Jan 19. PMID: 33472056
Yang, C.K., Kashyap, D.R., Kowalczyk, D.A., Rudner, D.Z., Wang, X., Gupta, D., Dziarski*, R. (2021) Respiratory chain components are required for peptidoglycan recognition protein-induced thiol depletion and killing in Bacillus subtilis and Escherichia coli. Scientific Reports. 2021 Jan 8;11(1):64. doi: 10.1038/s41598-020-79811-z. PMID: 33420211
Brandão, H.B., Paul, P., van del Berg, A.A., Rudner, D.Z., Wang*, X., Mirny*, L.A. (2019) RNA polymerases as moving barriers to condensin loop extrusion. PNAS. 2019 Oct 8;116(41):20489-20499. doi: 10.1073/pnas.1907009116. Epub 2019 Sep 23. PMID: 31548377
Brunet, Y., Wang, X., Rudner*, D.Z. (2019) SweC and SweD are essential co-factors of the FtsEX-CwlO cell wall hydrolase complex in Bacillus subtilis. PLOS Genetics. 2019 Aug 22;15(8):e1008296. doi: 10.1371/journal.pgen.1008296. eCollection 2019 Aug. PMID: 31437162
Wang*, X., Hughes, A.C., Brandão, H.B., Walker, B., Lierz, C., Cochran, J.C., Oakley, M.G., Kruse, A.C., Rudner*, D.Z. (2018) In vivo evidence for ATPase-dependent DNA translocation by the Bacillus subtilis SMC condensin complex. Molecular Cell. 22018 Sep 6;71(5):841-847.e5. doi: 10.1016/j.molcel.2018.07.006. Epub 2018 Aug 9. PMID: 30100265
Ramírez-Guadiana, F.H., Meeske, A.J., Wang, X., Rodrigues, C.D.A., Rudner*, D.Z. (2017) The Bacillus subtilis germinant receptor GerA triggers premature germination in response to morphological defects during sporulation. Molecular Microbiology. 2017 Sep;105(5):689-704. doi: 10.1111/mmi.13728. Epub 2017 Jul 6. PMID: 28605069
Wang*, X., Brandão H.B., Le, T.B., Laub, M.T., Rudner*, D.Z. (2017) Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus. Science. 2017 Feb 3;355(6324):524-527. doi: 10.1126/science.aai8982. PMID: 28154080
Harvard Medical School
Pang, T., Wang, X., Lim, H.C., Bernhardt, T.G., Rudner, D.Z. (2017) The nucleoid occlusion factor Noc controls DNA replication initiation in Staphylococcus aureus. PLOS Geneticsics. 2017 Jul 19;13(7):e1006908. doi: 10.1371/journal.pgen.1006908. eCollection 2017 Jul. PMID: 28723932
Rodrigues, C.D., Ramírez-Guadiana, F.H., Meeske, A.J., Wang, X., Rudner, D.Z. (2016) GerM is required to assemble the basal platform of the SpoIIIA-SpoIIQ transenvelope complex during sporulation in Bacillus subtilis. Molecular Microbiology. 2016 Oct;102(2):260-273. doi: 10.1111/mmi.13457. Epub 2016 Jul 22. PMID: 27381174
Wang*, X., Montero Llopis, P. (2016) Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation. Methods in Molecular Biology. 2016;1431:275-87. doi: 10.1007/978-1-4939-3631-1_19. PMID: 27283315
Wang, X., Le, T.B., Lajoie, B.R., Dekker, J., Laub, M.T., Rudner, D.Z.(2015). Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtilis. Genes & Development. 2015 Aug 1;29(15):1661-75. doi: 10.1101/gad.265876.115. PMID: 26253537
Wang, X., Rudner, D.Z. (2014) Spatial organization of bacterial chromosomes. Current Opinion in Microbiology. 2014 Dec;22:66-72. doi: 10.1016/j.mib.2014.09.016. PMID: 25460798 [review].
Wang, X., Montero Llopis, P., Rudner, D.Z. (2014) Bacillus subtilis chromosome organization oscillates between two distinct patterns. PNAS. 2014 Sep 2;111(35):12877-82. doi: 10.1073/pnas.1407461111. Epub 2014 Jul 28. PMID: 25071173
Broedersz, C.P., Wang, X., Meir, Y., Loparo, J.J., Rudner, D.Z., Wingreen, N.S. (2014) Condensation and localization of the partitioning protein ParB on the bacterial chromosome. PNAS. 2014 Jun 17;111(24):8809-14. doi: 10.1073/pnas.1402529111. Epub 2014 Jun 3. PMID: 24927534
Graham, T.G., Wang, X., Song, D., Etson, C.M., van Oijen, A.M., Rudner, D.Z., Loparo, J.J. (2014) ParB spreading requires DNA bridging. Genes & Development. 2014 Jun 1;28(11):1228-38. doi: 10.1101/gad.242206.114. Epub 2014 May 14. PMID: 24829297
Pimentel, B., Nair, R., Bermejo-Rodríguez, C., Preston, M.A., Agu, C.A., Wang, X., Bernal, J.A., Sherratt, D.J., de la Cueva-Méndez, G. (2014) Toxin Kid uncouples DNA replication and cell division to enforce retention of plasmid R1 in Escherichia coli cells. PNAS. 2014 Feb 18;111(7):2734-9. doi: 10.1073/pnas.1308241111. Epub 2014 Jan 21. PMID: 24449860
Wang, X., Tang, O.W., Riley, E.P., Rudner, D.Z. (2014) The SMC condensin complex is required for origin segregation in Bacillus subtilis. Current Biology. 2014 Feb 3;24(3):287-92. doi: 10.1016/j.cub.2013.11.050. Epub 2014 Jan 16. PMID: 24440393
Wang, X., Montero Llopis, P., Rudner, D.Z.(2013) Organization and segregation of bacterial chromosomes. Nature Reviews Genetics. 2013 Mar;14(3):191-203. doi: 10.1038/nrg3375. Epub 2013 Feb 12. PMID: 23400100 [review]
Yardimci, H., Wang, X., Loveland, A.B., Tappin, I., Rudner, D.Z., Hurwitz, J., van Oijen, A.M., Walter, J.C. (2012) Bypass of a protein barrier by a replicative DNA helicase. Nature. 2012 Dec 13;492(7428):205-9. doi: 10.1038/nature11730. Epub 2012 Nov 28. PMID: 23201686
Jackson, D., Wang, X., Rudner D.Z. (2012) Spatio-temporal organization of replication in bacteria and eukaryotes (nucleoids and nuclei). Cold Spring Harbor Perspectives in Biology. 2012 Aug 1;4(8):a010389. doi: 10.1101/cshperspect.a010389. PMID: 22855726 [review]
University of Oxford
Wang, X., Lesterlin, C., Reyes-Lamothe, R., Ball, G., Sherratt, D.J. (2011) Replication and segregation of an Escherichia coli chromosome with two replication origins. PNAS. 2011 Jun 28;108(26):E243-50. doi: 10.1073/pnas.1100874108. Epub 2011 Jun 13. PMID: 21670292
Wang, X., Sherratt, D.J. (2010) Independent segregation of the two arms of the Escherichia coli ori region requires neither RNA synthesis nor MreB dynamics. Journal of Bacteriology. 2010 Dec;192(23):6143-53. doi: 10.1128/JB.00861-10. Epub 2010 Oct 1. PMID: 20889756
Liu, X., Wang, X., Reyes-Lamothe, R., Sherratt, D.J. (2010) Replication-directed sister chromosome alignment in Escherichia coli. Molecular Microbiology. 2010 Mar;75(5):1090-7. doi: 10.1111/j.1365-2958.2009.06791.x. PMID: 20487299
Wang, X., Reyes-Lamothe, R., Sherratt, D.J. (2008) Modulation of Escherichia coli sister chromosome cohesion by topoisomerase IV. Genes & Development. 2008 Sep 1;22(17):2426-33. doi: 10.1101/gad.487508. PMID: 18765793
Wang, X., Reyes-Lamothe, R., Sherratt, D.J. (2008) Visualizing genetic loci and molecular machines in living bacteria. Biochemical Society Transactions. 2008 Aug;36(Pt 4):749-53. doi: 10.1042/BST0360749. PMID: 18631152 [review]
Reyes-Lamothe, R., Wang, X., Sherratt, D.J. (2008) Escherichia coli and its chromosome. Trends in Microbiology. 2008 May;16(5):238-45. doi: 10.1016/j.tim.2008.02.003. Epub 2008 Apr 9. PMID: 18406139 [review]
Wang, X., Liu, X., Possoz, C., Sherratt, D.J. (2006). The two Escherichia coli chromosome arms locate to separate cell halves. Genes & Development. 2006 Jul 1;20(13):1727-31. doi: 10.1101/gad.388406. PMID: 16818605
Wang, X., Possoz, C., Sherratt, D.J. (2005). Dancing around the divisome: asymmetric chromosome segregation in Escherichia coli. Genes & Development. 2005 Oct 1;19(19):2367-77. doi: 10.1101/gad.345305. PMID: 16204186