Publications

Indiana University

* corresponding author, † these authors contributed equally.

Sanath-Kumar†, R., Rahman†, A., Ren, Z, Reynolds, I.P., Augusta, L., Fuqua, C, J. Weisberg*, A.J., Wang*, X. (2025) Linear dicentric bacterial chromosomes in Agrobacterium tumefaciens natural isolates reveal common constraints for replicon fusion. mBio. 2025 May 20:e0104625. doi: 10.1128/mbio.01046-25. PMID: 40391973. PDF Supplement

Mishra, A., Jackson, A., Wang, X., Kearns*, D.B. (2025) The SinR•SlrR heteromer attenuates transcription of a long operon of flagellar genes in Bacillus subtilis. Journal of Molecular Biology. 2025 Apr 3:169123. doi: 10.1016/j.jmb.2025.169123. PMID: 40187681. PDF Supplement

Ren†, Z., Way†, L.E., Wang*, X. (2025) SMC translocation is unaffected by an excess of nucleoid associated proteins in vivo. Scientific Reports. 15, 2447. doi: 10.1038/s41598-025-86946-4. PDF Supplement

Liao, Q., Wang*, X. (2025) Using Chromosome Conformation Capture Combined with Deep Sequencing (Hi-C) to Study Genome Organization in Bacteria. High Throughput Gene Screening: Methods and Protocols. Methods in Molecular Biology. 2025:2866:231-243. doi: 10.1007/978-1-0716-4192-7_13. PDF

Baglivo*, I., Malgieri, G., Roop, R.M. 2nd, Barton, I.S., Wang, X., Russo, V., Pirone, L., Pedone, E.M., Pedone*, P.V. (2024) MucR protein: Three decades of studies have led to the identification of a new H-NS-like protein. Mol Microbiol. 2024 Apr 15. doi: 10.1111/mmi.15261. PMID: 38619026. PDF

Mishra, A., Hughes, A.C., Amon, J.D., Rudner, D.Z., Wang, X., Kearns*, D.B. (2024) SwrA-mediated multimerization of DegU and an upstream activation sequence enhance flagellar gene expression in Bacillus subtilis. Journal of Molecular Biology. 2024 Feb 15;436(4):168419. doi: 10.1016/j.jmb.2023.168419. Epub 2023 Dec 21. PMID: 38141873; PMCID: PMC11462632. PDF Supplement

Barton, I.S., Ren, Z., Cribb, C.B., Pitzer, P.E., Baglivo, I., Martin, D.W., Wang*, X., Roop*, R.M. (2023) Brucella MucR acts as an H-NS-like protein to silence virulence genes and structure the nucleoid. mBio. 2023 Oct 17:e0220123. doi: 10.1128/mbio.02201-23. PDF

Molina†, M., Way†, L.E., Ren, Z., Liao, Q., Guerra, B. Shields, B., Wang*, X., Kim*, H. (2023) A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments. Cell Reports Methods. 2023 Oct 3:100614. doi: 10.1016/j.crmeth.2023.100614. PDF Supplement Preview

Ren†, Z., Takacs†, C.N., Brandão, H.B., Jacobs-Wagner*, C., Wang*, X. (2023) Organization and replicon interactions within the highly segmented genome of Borrelia burgdorferi. PLoS Genet. 2023 Jul 26;19(7):e1010857. doi: 10.1371/journal.pgen.1010857. PDF Supplement

Takacs, C.N., Wachter†, J., Xiang†, Y., Ren†, Z, Karaboja, X, Scott, M., Stoner, M.R., Irnov, I., Jannetty, N., Rosa, P.A., Wang*, X., Jacobs-Wagner*, C. (2022) Polyploidy, regular patterning of genome copies, and alternative control of DNA partitioning in the Lyme disease spirochete. Nat Commun. 2022 Nov 22;13(1):7173. doi: 10.1038/s41467-022-34876-4. PDF Supplement Correction

Lamanna, M.M., Manzoor, I., Joseph, M., Ye, Z.A., Benedet, M., Zanardi, A., Ren, Z., Wang, X., Massidda, O., Tsui*, H.C.T., Winkler*, M.E. (2022) Roles of RodZ and Class A PBP1b in the Assembly and Regulation of the Peripheral Peptidoglycan Elongasome in Ovoid-Shaped Cells of Streptococcus pneumoniae D39. Mol Microbiol. 2022 Oct;118(4):336-368. doi: 10.1111/mmi.14969. PDF

Liao†, Q., Ren†, Z., Wiesler, E.E., Fuqua, C., Wang*, X. (2022) A dicentric bacterial chromosome requires XerC/D site-specific recombinases for resolution. Current Biology. 2022 Aug 22;32(16):3609-3618.e7. doi: 10.1016/j.cub.2022.06.050. PDF Supplement Dispatch

Sanchez, S., Snyder, E.V., Wang, X., Kearns*, D.B. (2022) Identification of genes required for swarming motility in Bacillus subtilis using transposon mutagenesis and high-throughput sequencing (TnSeq). J Bacteriol. 2022 Jun 21;204(6):e0008922. doi: 10.1128/jb.00089-22. PDF Supplement

Montero Llopis P., Stephansky R., Wang*, X. (2022) High-throughput imaging of Bacillus subtilis. Methods in Molecular Biology. 2022;2476:277-292. doi: 10.1007/978-1-0716-2221-6_19. PDF

Karaboja, X., Wang*, X. (2022) HBsu is required for the initiation of DNA replication in Bacillus subtilis. J Bacteriol. 2022 Aug 16;204(8):e0011922. doi: 10.1128/jb.00119-22. PDF Supplement Commentary

Ren, Z., Liao, Q., Barton, I.S., Wiesler, E.E., Fuqua, C., Wang*, X. (2022) Centromere interactions promote the maintenance of the multipartite genome in Agrobacterium tumefaciens. mBio. 2022 Jun 28;13(3):e0050822. doi: 10.1128/mbio.00508-22. PDF Supplement

Ren, Z., Liao, Q., Karaboja, X., Barton, I.S., Schantz, E.G., Mejia-Santana, A., Fuqua, C., Wang*, X. (2022) Conformation and dynamic interactions of the multipartite genome in Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 2022 Feb 8;119(6):e2115854119. doi: 10.1073/pnas.2115854119. PDF Supplement

Dobihal, G.S., Flores-Kim, J., Roney, I.J., Wang, X., Rudner*, D.Z. (2022) The WalR-WalK signaling pathway modulates the activities of both CwlO and LytE through control of the peptidoglycan deacetylase PdaC in Bacillus subtilis. J Bacteriol. 2022 Feb 15;204(2):e0053321. doi: 10.1128/JB.00533-21. PDF Supplement

Brandão†, H.B., Ren†, Z., Karaboja, X., Mirny*, L.A., Wang*, X. (2021) DNA-loop extruding SMC complexes can traverse one another in vivo. Nat Struct Mol Biol. 2021 Aug;28(8):642-651. doi: 10.1038/s41594-021-00626-1. PDF Supplement Cover

Karaboja, X., Ren, Z., Brandão, H.B., Paul, P., Rudner*, D.Z., Wang*, X. (2021) XerD unloads bacterial SMC complexes at the replication terminus. Molecular Cell. 2021 Feb 18;81(4):756-766.e8. doi: 10.1016/j.molcel.2020.12.027. PDF Supplement

Yang, C.K., Kashyap, D.R., Kowalczyk, D.A., Rudner, D.Z., Wang, X., Gupta, D., Dziarski*, R. (2021) Respiratory chain components are required for peptidoglycan recognition protein-induced thiol depletion and killing in Bacillus subtilis and Escherichia coli. Sci Rep. 2021 Jan 8;11(1):64. doi: 10.1038/s41598-020-79811-z. PDF

Brandão, H.B., Paul, P., van del Berg, A.A., Rudner, D.Z., Wang*, X., Mirny*, L.A. (2019) RNA polymerases as moving barriers to condensin loop extrusion. Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20489-20499. doi: 10.1073/pnas.1907009116. PDF Supplement

Brunet, Y., Wang, X., Rudner*, D.Z. (2019) SweC and SweD are essential co-factors of the FtsEX-CwlO cell wall hydrolase complex in Bacillus subtilis. PLoS Genet. 2019 Aug 22;15(8):e1008296. doi: 10.1371/journal.pgen.1008296. PDF

Wang*, X., Hughes, A.C., Brandão, H.B., Walker, B., Lierz, C., Cochran, J.C., Oakley, M.G., Kruse, A.C., Rudner*, D.Z. (2018) In vivo evidence for ATPase-dependent DNA translocation by the Bacillus subtilis SMC condensin complex. Molecular Cell. 2018 Sep 6;71(5):841-847.e5. doi: 10.1016/j.molcel.2018.07.006. PDF Supplement

Ramírez-Guadiana, F.H., Meeske, A.J., Wang, X., Rodrigues, C.D.A., Rudner*, D.Z. (2017) The Bacillus subtilis germinant receptor GerA triggers premature germination in response to morphological defects during sporulation. Mol Microbiol. 2017 Sep;105(5):689-704. doi: 10.1111/mmi.13728. PDF Supplement

Wang*, X., Brandão H.B., Le, T.B., Laub, M.T., Rudner*, D.Z. (2017) Bacillus subtilis SMC complexes juxtapose chromosome arms as they travel from origin to terminus. Science. 2017 Feb 3;355(6324):524-527. doi: 10.1126/science.aai8982. PDF Supplement perspective

Harvard Medical School

Pang, T., Wang, X., Lim, H.C., Bernhardt, T.G., Rudner, D.Z. (2017) The nucleoid occlusion factor Noc controls DNA replication initiation in Staphylococcus aureus. PLOS Genetics. 13(7): e1006908. PDF Supplement Highlight

Rodrigues, C.D., Ramírez-Guadiana, F.H., Meeske, A.J., Wang, X., Rudner, D.Z. (2016) GerM is required to assemble the basal platform of the SpoIIIA-SpoIIQ transenvelope complex during sporulation in Bacillus subtilis. Mol Microbiol. 2016 Oct;102(2):260-273. PDF Supplement

Wang*, X., Montero Llopis, P. (2016) Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation. Methods Mol Biol. 2016;1431:275-87. PDF

Wang, X., Le, T.B., Lajoie, B.R., Dekker, J., Laub, M.T., Rudner, D.Z.(2015). Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtilis. Genes Dev. 29, 1661-1675. PDF Supplement News and Views

Wang, X., Rudner, D.Z. (2014) Spatial organization of bacterial chromosomes. Curr Opin Microbiol. 22, 66-72 [review]. PDF

Wang, X., Montero Llopis, P., Rudner, D.Z. (2014) Bacillus subtilis chromosome organization oscillates between two distinct patterns. Proc Natl Acad Sci U S A. 111 (35) 12877-12882. PDF Commentary

Broedersz, C.P., Wang, X., Meir, Y., Loparo, J.J., Rudner, D.Z., Wingreen, N.S. (2014) Condensation and localization of the partitioning protein ParB on the bacterial chromosome. Proc Natl Acad Sci U S A. 111 (24), 8809-14. PDF

Graham, T.G., Wang, X., Song, D., Etson, C.M., van Oijen, A.M., Rudner, D.Z., Loparo, J.J. (2014) ParB spreading requires DNA bridging. Genes Dev. 28(11), 1228-38. PDF Supplement Cover Perspective

Pimentel, B., Nair, R., Bermejo-Rodríguez, C., Preston, M.A., Agu, C.A., Wang, X., Bernal, J.A., Sherratt, D.J., de la Cueva-Méndez, G. (2014) Toxin Kid uncouples DNA replication and cell division to enforce retention of plasmid R1 in Escherichia coli cells. Proc Natl Acad Sci U S A. 111(7), 2734-9. PDF Supplement

Wang, X., Tang, O.W., Riley, E.P., Rudner, D.Z. (2014) The SMC condensin complex is required for origin segregation in Bacillus subtilis. Current Biology. 24(3), 287-92. PDF Supplement Dispatch

Wang, X., Montero Llopis, P., Rudner, D.Z.(2013) Organization and segregation of bacterial chromosomes. Nat Rev Genet. 14(3), 191-203. [review] PDF Cover

Yardimci, H., Wang, X., Loveland, A.B., Tappin, I., Rudner, D.Z., Hurwitz, J., van Oijen, A.M., Walter, J.C. (2012) Bypass of a protein barrier by a replicative DNA helicase. Nature. 492(7428), 205-9. PDF Supplement News and Views

Jackson, D., Wang, X., Rudner D.Z. (2012) Spatio-temporal organization of replication in bacteria and eukaryotes (nucleoids and nuclei). Cold Spring Harb Perspect Biol. 4(8). pii: a010389. doi: 10.1101/cshperspect.a010389. [review] PDF

University of Oxford

Wang, X., Lesterlin, C., Reyes-Lamothe, R., Ball, G., Sherratt, D.J. (2011) Replication and segregation of an Escherichia coli chromosome with two replication origins. Proc Natl Acad Sci U S A. 108 (26) E243–E250. PDF Supplement

Wang, X., Sherratt, D.J. (2010) Independent segregation of the two arms of the Escherichia coli ori region requires neither RNA synthesis nor MreB dynamics. J Bacteriol. 192, 6143-53. PDF Cover

Liu, X., Wang, X., Reyes-Lamothe, R., Sherratt, D.J. (2010) Replication-directed sister chromosome alignment in Escherichia coli. Mol Microbiol. 75, 1090-7. [Epub 2009 Jul 24] PDF Supplement

Wang, X., Reyes-Lamothe, R., Sherratt, D.J. (2008) Modulation of Escherichia coli sister chromosome cohesion by topoisomerase IV. Genes Dev. 22, 2426-2433. PDF Perspective

Wang, X., Reyes-Lamothe, R., Sherratt, D.J. (2008) Visualizing genetic loci and molecular machines in living bacteria. Biochem Soc Trans. 36, 749-753. [review] PDF

Reyes-Lamothe, R., Wang, X., Sherratt, D.J. (2008) Escherichia coli and its chromosome. Trends Microbiol. 16, 238-245. [review] PDF

Wang, X., Liu, X., Possoz, C., Sherratt, D.J. (2006). The two Escherichia coli chromosome arms locate to separate cell halves. Genes Dev. 20, 1727-1731. PDF Cover

Wang, X., Possoz, C., Sherratt, D.J. (2005). Dancing around the divisome: asymmetric chromosome segregation in Escherichia coli. Genes Dev. 19, 2367-2377. PDF